Device

Part:BBa_K323080

Designed by: Jernej Turnšek   Group: iGEM10_Slovenia   (2010-10-20)

CMV ATG cYFP link Zif268 link His stop - part of mammalian split/FRET system


iGEM 2010 team Slovenia used this part along with 4 others [BBa_K323005, BBa_K323021, BBa_K323029, BBa_323039] to exhibit FRET reconstitution on a predefined DNA scaffold in vivo by transfecting mammalian HEK293 cell line with these 5 plasmids. In particular this BioBrick is part of a FRET subsystem leading to split mCitrine reassembly. It consists of a C terminal fragment of mCitrine, a linker sequence and Zif268 zinc finger DNA binding domain. Reconstitution of a fluorescent protein was observed under confocal microscope. mCitrine was excited with 514nm Argon laser and emmision was detected in 520 to 580 nm window. Combining this 2-part subsystem with the other one, leading to mCerulean reassembly [BBa_K323021, BBa_K323029], FRET on a DNA program can be observed.


Click Experience to find out more!

Check out more at [http://2010.igem.org/Team:Slovenia Team Slovenia]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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